The Wnt signaling pathway is an ancient and evolutionarily conserved pathway that regulates crucial aspects of cell fate determination, cell migration, cell polarity, neural patterning and organogenesis during embryonic development. Aberrant regulation of the Wnt signaling pathway is a prevalent theme in cancer biology. The Hedgehog (Hh) pathway is a major regulator of many fundamental processes in vertebrate embryonic development including stem cell maintenance, cell differentiation, tissue polarity and cell proliferation. Constitutive activation of the Hh pathway leading to tumorigenesis is seen in basal cell carcinomas and medulloblastoma. A variety of other human cancers, including brain, gastrointestinal, lung, breast and prostate cancers, also demonstrate inappropriate activation of this pathway. Targeting the Hh signaling pathway provides a new and exciting therapeutic option for a broad variety of cancers. The Notch signaling pathway is a highly conserved cell signaling system present in most multicellular organisms. The Notch signaling cascade is critical for cell proliferation, differentiation, development and homeostasis. Deregulated Notch signaling is found in various diseases, such as T-cell leukemia, breast cancer, prostate cancer, colorectal cancer and lung cancer as well as central nervous system (CNS) malignancies, CADASIL, Alagille syndrome, spondylocostal dysostosis, etc.

Wnt/Hedgehog/Notch Compound Library from TargetMol, a unique collection of 237 Wnt/Hedgehog/Notch signaling targeted compounds, can be used for research in Wnt/Hedgehog/Notch signaling and related drug screening (high throughput and high content screening).